镇惊温胆汤对PTSD模型大鼠ORX受体基因表达的调节作用

目的:研究镇惊温胆汤对创伤后应激障碍模型大鼠皮质边缘系统中食欲素(Orexin,ORX)受体OX1R和OX2R基因表达的调节以及血清中皮质酮和神经元特异性烯醇化酶的调节。方法:采用单次延长应激法复制创伤后应激障碍(Post traumatic stress disorder,PTSD)模型大鼠,采用实时荧光定量聚合酶链反应(RT-PCR)技术分析大鼠脑区中OX1R及OX2R的变化,采用酶联免疫吸附试验(Elisa)分析大鼠血清中皮质酮和神经元特异性烯醇化酶的变化。结果:镇惊温胆汤组提高了前额叶皮质(PFC)及杏仁核(AMY)中OX1R、OX2R的基因表达,降低了在血清中皮质酮和神经元特异性烯醇化酶的含量,有极显著的差异(P0.01),对海马(HIP)中OX1R、OX2R的基因表达有微弱的上调作用。结论:中药复方镇惊温胆汤可以调控大鼠ORX系统OX1R及OX2R受体和血清中皮质酮和神经元特异性烯醇化酶含量,能够改善大脑兴奋-抑制功能失衡状态,从而有效缓解PTSD不寐、善惊等病理状态。

Effect of Zhenjingwendan Decoction on Gene Expression of ORX Receptor in PTSD Rats

ABSTRACT Objective: To study the regulation of Zhenjingwendan decoction on corticosterone and neuron-specific enolase in serum and the expression of ORX receptor orexin1 receptor (OX1R) and orexin2 receptor (OX2R) in cortical limbic system of rats with post-traumatic stress disorder. Methods: PTSD model rats were established by single prolonged stress. The changes of OX1R and OX2R in brain region of rats were analyzed by real time PCR technique, and the changes of corticosterone and neuron specific enolase in serum were analyzed by Elisa. Results: The Zhenjingwendan decoction group increased the expression of OX1R and OX2R genes in the prefrontal cortex (PFC) and amygdala (AMY), reduced the levels of corticosterone and neuron-specific enolase in the serum, with significantly difference between the two groups (P<0.01). At the same time, the gene expression of OX1R and OX2R in hippocampus (HIP) was increased slowly. Conclusion: Zhenjingwendan decoction can regulate the content of corticosterone and neuron-specific enolase in rat serum and OX1R and OX2R receptors in ORX system, improve the imbalance of brain excitation-inhibition function, and alleviate the pathological state of insomnia and susceptible to fright effectively.