CD80、CD86在鼻咽癌中的表达及其临床病理意义

目的:研究CD80、CD86在鼻咽癌中的表达变化及其临床病理意义。方法:选择2014年10月至2018年10月本院接诊的鼻咽癌确诊患者64例纳入研究,依据鼻咽癌复发情况分复发组(n=30)和未复发组(n=34);同期选取正常鼻粘膜活检组织33例作为正常对照组,采用SP免疫组化法检测鼻咽癌患者癌组织或正常鼻粘膜活检组织CD80、CD86蛋白的表达,并经Spearman相关性分析法分析CD80、CD86蛋白表达与鼻咽癌恶化程度的相关性。结果:鼻咽癌的癌组织CD80、CD86蛋白均呈高表达,阳性表达主要定位于细胞膜、细胞质,与肿瘤临床TNM分期、淋巴结转移均显著相关(P0.05)。复发组、未复发组肿瘤组织中的mRNA(ARD1、Ptch1、Survivin)表达显著高于对照组,且复发组高于未复发组,差异有统计学意义(P0.05)。Spearman相关性分析显示CD80、CD86蛋白表达与鼻咽癌细胞侵袭能力呈显著正相关(r=0.403、0.547,P0.05)。结论:鼻咽癌的癌组织内CD80、CD86蛋白均呈高表达,与鼻咽癌的临床分期、淋巴结转移及放疗预后关系密切,可能作鼻咽癌临床诊治及预后评估的重要参考指标。

Expression and Clinical Significance of CD80 and CD86 in the Nasopharyngeal Carcinoma

ABSTRACT Objective: To study the expression changes of CD80 and CD86 in nasopharyngeal carcinoma and their effects on tumor cell proliferation and invasion ability. Methods: 64 cases of patients with confirmed nasopharyngeal cancer who were admitted to our hospital from October 2014 to October 2018 were enrolled in the study. All patients were divided into recurrent group (n=30) and non-recurred group (n=34) according to the recurrence of nasopharyngeal carcinoma; in the same period, 33 cases of normal nasal mucosa biopsy were selected as normal control group. The expression of CD80 and CD86 protein in cancer tissues of patients with nasopharyngeal carcinoma was analyzed by SP immunohistochemistry. The correlation between CD80 and CD86 protein expression and the degree of deterioration of nasopharyngeal carcinoma was analyzed by Spearman correlation analysis. Results: CD80 and CD86 proteins were highly expressed, and showed brown positive staining in the cell membrane and cytoplasm. The positive expression of CD80 and CD86 protein in nasopharyngeal carcinoma tissues was associated with clinical TNM staging and lymph node metastasis (P<0.05); The mRNA (ARD1, Ptch1, Survivin) expression in the recurrent and non-recurrent groups was significantly higher than that in the control group, and the recurrence group was higher than the non-recurrent group (P<0.05). Spearman correlation analysis showed that CD80 and CD86 protein expression were positively correlated with the invasion ability of nasopharyngeal carcinoma cells (r=0.403, 0.547, P<0.05). Conclusion: CD80 and CD86 proteins are highly expressed in cancer tissues of nasopharyngeal carcinoma. At the same time, the expression of CD80 and CD86 protein in tumor tissues is closely related to the clinical stage, lymph node metastasis and prognosis of nasopharyngeal carcinoma, which can be used as an important index for clinical diagnosis and treatment of nasopharyngeal carcinoma.