结直肠癌COX-2表达及其与临床病理特征的相关性分析

目的:探讨结直肠癌环氧化酶-2(cyclooxygenase-2,COX-2)的表达及其与临床病理特征的相关性。方法:选择2017年8月～2019年6月在本院外科手术诊治的结直肠癌患者60例,取所有患者的病灶组织标本与癌旁组织标本,采用PCR与免疫组化法检测COX-2 mRNA与蛋白表达情况,分析其与患者的临床病理特征的相关性。结果:直肠癌组织COX-2 mRNA与蛋白表达阳性率分别为63.3%和55.0%,显著高于癌旁组织的20.0%和16.7%(P0.05)。随着结直肠癌的病理分期及分化程度的增加、淋巴结转移的发生,直肠癌组织的COX-2 mRNA、蛋白表达阳性率显著升高(P0.05)。Spearman等级相关分析显示直肠癌组织的COX-2 mRNA、蛋白表达阳性率与临床分期、组织学分化与淋巴结转移都存在显著相关性(P0.05)。Cox模型多因素分析显示临床分期、组织学分化与淋巴结转移都是影响COX-2蛋白表达的主要因素(P0.05)。结论:结直肠癌COX-2的mRNA与蛋白都呈现高表达,与患者的临床分期、组织学分化与淋巴结转移显著相关。

Correlation of COX-2 Expression with the Clinicopathological Features of Colorectal Cancer

ABSTRACT Objective: To investigate the expression of cyclooxygenase-2 (COX-2) and its correlation with the clinicopathological features of colorectal cancer. Methods: From August 2017 to June 2019, 60 patients with colorectal cancer who underwent surgery in our hospital were enrolled. The specimens of the lesions and adjacent tissues were collected from all patients. The expression of mRNA and protein of COX-2 were detected by PCR and immunohistochemistry, the clinicopathological features of the patients were investigated and were given correlation analysis. Results: The positive rates of COX-2 mRNA and protein expression in lesion tissues were 63.3% and 55.0%, respectively, which were significantly higher than those in adjacent tissues (20.0% and 16.7%)(P<0.05). With the increased of pathological stage, degree of differentiation and lymph node metastasis of colorectal cancer, the positive rates of COX-2 mRNA and protein expression in lesion tissues were increased significantly (P<0.05). Spearman rank correlation analysis showed that the positive rate of COX-2 mRNA and protein expression in the lesion tissues were correlated with clinical stage, histological differentiation and lymph node metastasis (P<0.05). Multivariate analysis of Cox model showed that clinical stage, histological differentiation and lymph node metastasis were the main factors affectedCOX-2 protein expression (P<0.05). Conclusion: The mRNA and protein of COX-2 in colorectal cancer are highly expressed, which aresignificantly correlated with clinical stage, histological differentiation and lymph node metastasis.