巨噬细胞装载纳米颗粒用于药物递送

目的:活细胞药物递送系统具有主动靶向至肿瘤部位,防止被免疫系统清除等诸多优势。本文提供了一种巨噬细胞负载纳米颗粒的递送方法,并探讨不同载药量对巨噬细胞的活性以及运动性的影响。方法:通过超声乳化法制备包载阿霉素的DOX@PLGA纳米颗粒。纳米粒度分析仪测量粒径和表面电位,透射电镜观察纳米颗粒形态。将DOX@PLGA纳米颗粒与巨噬细胞共同孵育,即得到负载DOX@PLGA纳米颗粒的巨噬细胞用以药物递送。然后通过CCK-8法、LDH法以及细胞迁移实验检测不同载药量情况下细胞活力水平、细胞损伤程度以及细胞运动性。结果:制备的DOX@PLGA纳米颗粒呈圆形或椭圆形,粒径为109.2±2.3 nm;表面电位为-45.0±2.0 m V;载药量为4.61%。当单个巨噬细胞负载0.15 pg DOX时细胞存活率为:71.5±4.4(%);细胞损伤率为:26.3±1.8(%);迁移率为:61.6±5.7(%)。结论:成功制备巨噬细胞负载DOX@PLGA纳米颗粒的递药系统,载药量适当的情况下载体细胞依然具有良好的活性和运动性。

Macrophages Loaded with Nanoparticles for Drug Delivery

ABSTRACT Objective: The live cell drug delivery system has many advantages, such as actively targeting to the tumor site, preventing it from being cleared by the immune system and so on. This study provides a method for delivering macrophage-loaded nanoparticles and explores the effects of different drug loadings on the activity and motility of macrophages. Methods: DOX@PLGA nanoparticles containing adriamycin were prepared by phacoemulsification. The particle size and zeta potential were measured by nanoparticle size analyzer, and the morphology of nanoparticles was observed by a transmission electron microscopy. Incubate the nanoparticles with macrophages to obtain macrophages loaded with DOX@PLGA nanoparticles for drug delivery. Then CCK-8 method, LDH method and cell migration experiments were used to detect the level of cell viability, cell damage degree and cell motility under different drug loading conditions. Results: The prepared DOX @ PLGA nanoparticles were round or elliptical; the size was 109.2±2.3 nm; the zeta potential was -45.0±2.0 mV. When a single macrophage was loaded with 0.15 pg DOX, the cell survival rate was 71.5±4.4(%); the cell damage rate was 26.3±1.8(%); and the migration rate was 61.6±5.7(%).Conclusion: The delivery system of DOX@PLGA nanoparticles loaded with macrophages was successfully prepared. Carrier cells with appropriate drug loading still have favorable activity and mobility.