|
|||||
|
|
| 高效液相色谱-串联质谱分析微量格尔德霉素类似物 | |
| 引用本文： | 倪四阳,张侃,王玉成,赫卫清,王以光,贺玖明,武临专.高效液相色谱-串联质谱分析微量格尔德霉素类似物[J].生物工程学报,2009,25(6):847-853. |
| 作者姓名： | 倪四阳 张侃 王玉成 赫卫清 王以光 贺玖明 武临专 |
| 作者单位： | 1. 中国医学科学院/北京协和医学院医药生物技术研究所卫生部抗生素生物工程重点实验室,北京,100050 2. 中国医学科学院/北京协和医学院医药生物技术研究所卫生部抗生素生物工程重点实验室,北京,100050;沈阳药科大学制药工程学院,沈阳,110016 3. 中国医学科学院/北京协和医学院药物研究所,北京,100050 |
| 摘 要： | 安莎类抗生素例如利福霉素和安丝菌素,通常由一组化学结构相似的组分组成。格尔德霉素为苯安莎类抗生素,已经发现4个组分。本研究采用高效液相色谱-串联质谱方法对格尔德霉素(GDM)制品中的微量组分进行了分析,发现5个新的和1个已知的GDM类似物。依据质谱数据、结合GDM生物合成机制,对6个GDM类似物的化学结构进行了推测:分子式为C29H42N2O10的新化合物3个,分别为GDM安莎链上C2-C3、C4-C5和C8-C9之间的C-C双键变为单键并同时单羟基化的GDM衍生物;分子式为C28H38N2O8的新化合物2个,其中1个为17(或12,或4)-去甲氧基格尔德霉素,另1个为4,5-双氢-10,11-脱水-17-去甲基-17-羟基格尔德霉素;分子式为C29H42N2O9的已知化合物1个,为4,5-双氢格尔德霉素。这些GDM类似物的发现有助于加深对GDM生物合成的认识,并对通过基因阻断、组合生物合成技术获得GDM衍生物的研究有启示作用。 |
| 关 键 词： | 高效液相色谱-串联质谱 格尔德霉素 格尔德霉素类似物 生物合成 |
| 收稿时间： | 1/8/2009 12:00:00 AM |
Analysis of geldanamycin analogues in trace amounts by LC-MS/MS |
|
| Siyang Ni,Kan Zhang,Yucheng Wang,Weiqing He,Yiguang Wang,Jiuming He and Linzhuan Wu.Analysis of geldanamycin analogues in trace amounts by LC-MS/MS[J].Chinese Journal of Biotechnology,2009,25(6):847-853. | |
| Authors: | Siyang Ni Kan Zhang Yucheng Wang Weiqing He Yiguang Wang Jiuming He and Linzhuan Wu |
| Institution: | Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China; School of Pharmaceutical Engineering, Shenyang Pharmaceuti;Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China |
| Abstract: | Ansamycins,such as rifamycin and ansamitocin,usually consist of a group of structural similar components.Geldanamycin,a benzenic ansamycin,has been found to consist of four structural similar components.We analyzed the geldanamycin(GDM) preparation from Streptomyces hygroscopicus 17997 by LC-ESI(+)-MS/MS,and discovered five novel and one known GDM analogues in trace amounts.Based on the ESI(+)-MS/MS spectra of these GDM analogues,and the present understanding of GDM biosynthesis,we proposed the possible che... |
| Keywords: | LC-MS/MS geldanamycin geldanamycin analogues biosynthesis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《生物工程学报》浏览原始摘要信息 | |
| 点击此处可从《生物工程学报》下载免费的PDF全文 | |