First Report of Aster Yellows Phytoplasma in Goldenrain Tree (Koelreuteria paniculata) in Korea |
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| Authors: | Seralathan Kamala‐Kannan Seung‐Moon Park Byung‐Taek OH Hyung‐Moo Kim Kui‐Jae Lee |
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| Institution: | 1. Authors’ addresses: Division of Biotechnology, College of Environmental & Bioresource Sciences, Chonbuk National University, Jeonju 561‐756, Korea;2. Division of Bioresources Science, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 561‐756, Korea (correspondence to Kui‐Jae Lee. E‐mail: kuijael@chonbuk.ac.kr) |
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| Abstract: | Aster yellows phytoplasma was detected for the first time in goldenrain tree (Koelreuteria paniculata) growing in Sinpyeong‐myeon, Jeollabuk‐do, South Korea. DNA was extracted from the infected leaf samples and part of the 16S rDNA, rp operon and tuf gene were amplified using R16F2n/R2 and gene‐specific primers. The sequence analysis showed that the phytoplasma was closely related (99%) to members of the Aster Yellows (AY) group, and belonging to 16Sr I, subgroup B. Moreover, the 16S rDNA sequences of the isolate showed 88–96% identity with members of other 16Sr and undesignated groups. Based on the sequence identity and phylogenetic studies, it was confirmed that phytoplasma infecting goldenrain tree in South Korea belongs to the AY group. |
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| Keywords: | aster yellows group goldenrain stunt phytoplasma 16S rDNA tuf gene rp operon South Korea |
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