| 禽多杀性巴氏杆菌粘附蛋白Cp39的交叉免疫保护作用 |
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| 引用本文： | 恩特马克?布拉提白,彭清忠,严芳,何翠,吾鲁木汗?那孜尔别克.禽多杀性巴氏杆菌粘附蛋白Cp39的交叉免疫保护作用[J].微生物学报,2010,50(3):360-366. |
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| 作者姓名： | 恩特马克?布拉提白 彭清忠 严芳 何翠 吾鲁木汗?那孜尔别克 |
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| 作者单位： | 吉首大学生物资源与环境科学学院,吉首,416000 |
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| 基金项目： | 湖南省教育厅基金项目（08C711）；吉首大学引进人才科研启动基金项目（2006031） |
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| 摘 要： | 【目的】比较体内外增殖禽多杀性巴氏杆菌荚膜蛋白、天然粘附蛋白Cp39和重组粘附蛋白rCp39对小鼠的交叉保护作用。【方法】用NaCl提取法制备鸡胚尿囊液和DSA培养基增殖的C48-3株荚膜蛋白,并用电洗脱方法纯化Cp39蛋白,将rCp39蛋白以可溶形式表达在大肠杆菌BL21后,用Amylose Resin亲和层析柱纯化。分别以100μg剂量的鸡胚尿囊液增殖菌体荚膜蛋白、DSA培养基培养菌体荚膜蛋白、纯化的Cp39蛋白和rCp39蛋白通过皮下注射各试验组小鼠,生理盐水为对照组,第二次免疫后2周分别以A:1型菌C48-3株(6.7×102cfu)和A:3型菌C51-3株(1.1×103cfu)进行攻毒试验。采集免疫后小鼠血清,用ELISA法检测抗体水平,并计算免疫保护率,来评价4种抗原对小鼠的交叉保护效果。【结果】SDS-PAGE结果显示,体内外增殖禽多杀性巴氏杆菌荚膜蛋白的条带和分子量相似,且体内外表达的Cp39蛋白的分子量相同;ELISA结果表明Cp39免疫组小鼠和rCp39免疫组小鼠血清rCp39蛋白特异性抗体的水平显著高于其他两组(P0.05);保护试验表明,体外增殖菌体荚膜蛋白免疫组小鼠对同源C48-3株和异源C51-3株攻毒的保护率分别为100%和60%,鸡胚尿囊液增殖菌体荚膜蛋白免疫组小鼠、Cp39免疫组小鼠和rCp39免疫组小鼠对同源C48-3株和异源C51-3株攻毒的保护率分别为100%和80%。【结论】粘附蛋白Cp39是禽多杀性巴氏杆菌荚膜蛋白中的主要交叉保护抗原,可以作为禽霍乱亚单位疫苗。
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| 关 键 词： | 关键词：多杀性巴氏杆菌 粘附蛋白 免疫原性 交叉保护 |
| 收稿时间： | 2009/8/20 0:00:00 |
| 修稿时间： | 2009/11/8 0:00:00 |
Cross-protective effect of the Cp39 adhesive proteins against avian Pasteurella multicida in mice |
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| Entomack Borrathybay,Qingzhong Peng,Fang Yan,Cui He and Wulumuhan Nazierbieke.Cross-protective effect of the Cp39 adhesive proteins against avian Pasteurella multicida in mice[J].Acta Microbiologica Sinica,2010,50(3):360-366. |
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| Authors: | Entomack Borrathybay Qingzhong Peng Fang Yan Cui He and Wulumuhan Nazierbieke |
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| Institution: | College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China;College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China;College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China;College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China;College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China |
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| Abstract: | Objective]We evaluated the cross-protective effect of a 39-kDa adhesive protein (Cp39), recombinant adhesive protein (rCp39), and capsular proteins from in vitro and in vivo grown avian P. multocida in mice. Methods] The Cp39 was purified by electroelution from capsular proteins of in vivo grown strain C48-3. The rCp39 was expressed in E. coli B21 as a soluble protein by IPTG inducing, and purified with pMAL~ TM protein fusion and purification system. Mice of each group were subcutaneously immunized twice... |
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| Keywords: | Pasteurella multocida adhesive protein immunogenicity cross-protection |
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