Different effects of lectins on the ligand binding of the NMDA receptors and sigma sites in rat brain hippocampus synaptic membranes |
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Authors: | Machaidze G G Mikeladze D |
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Institution: | (1) Department of Biophysical Chemistry, Biozentrum, University of Basel, CH-4056 Basel, Switzerland;(2) Department of Neurochemistry, I.S. Beritashvili Institute of Physiology, 380014 Tbilisi, Republic of Georgia |
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Abstract: | The effects of the lectins concanavalin A, WGA, ricin, abrin, and the mistletoe lectins from Viscum album MLI, MLII, and MLIII on the binding of ligands of the NMDA and sigma receptors in rat hippocampus synaptic plasma membranes were investigated. Binding of 3H]MK-801, 3H]glutamate, 3H]5,7-DCKA, and 3H]glycine to the membranes was decreased by 40-60% after addition of galactose-specific lectins (mistletoe lectins MLI, MLII, ricin, abrin) at concentrations of 0.01 mg/ml, but was not affected by the glucose- and mannose-specific lectin Con A, an acetylglucosamine-specific lectin WGA, or an acetylgalactosamine-specific lectin MLIII. The binding of 3H]SKF 10047 was decreased only in the presence of MLIII and did not change after addition of the other lectins. It is suggested that lectin-sensitive ligand binding sites of sigma- and NMDA receptors are located separately, and that the carbohydrate side chains of the sigma receptor do not participate in the modulation of the NMDA-receptor. |
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Keywords: | NMDA receptor sigma sites ligand binding mistletoe lectins ricin abrin |
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