Effects of experimental anaemia on intra-erythrocytic phosphate levels in rainbow trout, Oncorhynchus mykiss |
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Authors: | Adalberto L.,Val,Carl F.,Mazur&dagger ,Roberto H., de Salvo-Souza George K.,Iwama&dagger |
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Affiliation: | Instituto National de Pesquisas da Amazônia, 69083, Manaus, Amazonas, Brasil;Departmentof AnimalScience, The University of British Columbia, Vancouver, British Columbia V6T 2A2, Canada |
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Abstract: | The concentrations of intra-erythrocytic adenylates (ATP, ADP and AMP) and guanylates (GTP, GDP and GMP) were determined in rainbow trout subjected to 10% blood removal every 12 h for 96 h. Haemoglobin concentration, [Hb], decreased from 6·043±0·617 to 0·957 ± 0·195 g dl−1. This decrease in [Hb] was followed by a continuous increase in total organic phosphates, e.g. adenylates plus guanylates. Intra-erythrocytic NTP (ATP plus GTP) levels increased significantly after 48 h when haemoglobin concentration was 2·427 ± 0·256 g dl−1. Although a significant increase in GDP levels in animals with [Hb] less than 1·677 ± 0·235 g dl−1 was observed, the general increase in guanylate level was mainly due to the GMP which increased about 85-fold during the experimental period. It is suggested that the erythrocytes of anaemic rainbow trout have the capacity to increase NTP/Hb4 ratios which may represent an advantage for anaemic fish. |
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Keywords: | adenylates guanylates rainbow trout anaemia haematocrit |
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