A Study on the Prevalence of Bacteria that Occupy Nodules within Single Peanut Plants |
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Authors: | Jorge Angelini Fernando Ibáñez Tania Taurian María Laura Tonelli Lucio Valetti Adriana Fabra |
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Institution: | 1.Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales,Universidad Nacional de Río Cuarto,Río Cuarto,Argentina |
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Abstract: | In this study, bacteria hosted in root nodules of single plants of legume Arachis hypogaea L. (peanut) cv Tegua Runner growing at field were isolated. The collection of nodule isolates included both fast and slow
growing strains. Their genetic diversity was assessed in order to identify the more frequently rhizobial strain associated
to nodules from single plants. Molecular fingerprinting of 213 nodular isolates indicated heterogeneity, absence of a dominant
genotype and, therefore, of a unique strains highly competitive. Efficient nitrogen-fixing isolates were identified as Bradyrhizobium sp. by phylogenetic analysis of the sequences of their 16S rRNA genes. The genetic diversity of 68 peanut nodulating isolates
from all the collected plants was also analyzed. Considering their ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in this study indicate that the genetic diversity of rhizobia occupying
nodules from single plant is very high, without the presence of a dominant strain. Therefore, the identification of useful
peanut rhizobia for agricultural purposes requires strongly the selection, among the diverse population, of a very competitive
genotype in combination with a high-symbiotic performance. |
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