乙型肝炎病毒不同基因分型与淋巴细胞亚群分布、肝功能及脂代谢的关系

目的:探讨乙型肝炎病毒(HBV)不同基因分型与淋巴细胞亚群分布、肝功能及脂代谢的关系。方法:选择2016年10月-2017年12月在我院治疗的HBV患者130例,将患者进行HBV基因分型检查,根据不同基因分型将患者分为B型组(n=59)和C型组(n=71),采用实时荧光PCR法检测血清HBV-DNA载量,采用ADVIA2400全自动生化分析仪测定患者丙氨酸转氨酶(ALT)、白蛋白(ALB)、总胆红素(Tbil)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平,采用美国ACL-TOP700血凝仪检测凝血酶原时间(PT)。采用流式细胞仪测定不同基因分型患者CD3~+、CD4~+、CD8~+及CD4~+/CD8~+水平。结果:两组患者HBV-DNA载量、PT比较差异无统计学意义(P0.05);B型组患者ALT、ALB、TbiL水平均低于C型组(P0.05)。B型组患者CD3~+、CD4~+、CD4~+/CD8~+水平均高于C型组,CD8~+水平低于C型组(P0.05)。两组患者TC、TG、HDL-C和LDL-C水平比较差异无统计学意义(P0.05)。结论:不同基因分型对HBV患者病毒复制能力及脂代谢无明显影响,但C型HBV对患者免疫功能及肝功能损伤更严重。

Relationship between Different Genotypes of Hepatitis B Virus and Distribution of Lymphocyte Subsets, Liver Function and Lipid Metabolism

ABSTRACT Objective: To investigate the relationship between different genotypes of hepatitis B virus (HBV) and the distribution of lymphocyte subsets, liver function and lipid metabolism. Methods: 130 patients with HBV who were treated in our hospital from October 2016 to December 2017 were selected. The patients were examined by HBV genotypes, and according to different genotypes, the patients were divided into type B group (n=59) and type C group (n=71). The serum HBV-DNA load was measured by real-time fluorescence PCR. The levels of alanine aminotransferase (ALT), albumin (ALB), total bilirubin (Tbil), triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) of patients were determined using ADVIA2400 automatic biochemical analyzer, prothrombin time (PT) was detected using US ACL-TOP700 coagulation analyzer,and the levels of CD3⁺, CD4⁺, CD8⁺ and CD4⁺/CD8⁺ in patients with different genotypes were measured by flow cytometry. Results: There was no significant difference in HBV-DNA load and PT between the two groups (P>0.05). The levels of ALT, ALB and TbiL in type B group were lower than those in type C group(P<0.05). The levels of CD3⁺, CD4⁺ and CD4⁺/CD8⁺ in type B group were all higher than those in type C group, and the level of CD8⁺ was lower than that in type C group(P<0.05). There was no significant difference in the levels of TC, TG, HDL-C and LDL-C between the two groups (P>0.05). Conclusion: Different genotypes have no significant effect on viral replication and lipid metabolism in patients with HBV, however, C type HBV is more serious damage to immune function and liver function of patients.