胰岛素对2型糖尿病骨质疏松大鼠血清与骨OPG、RANKL表达的影响

目的:探讨胰岛素对2型糖尿病骨质疏松大鼠血清及骨OPG(osteoprotegerin)、RANKL(OPG receptor activator nuclear factork B)表达水平的影响。方法:以高脂高糖饲料喂养4周同时饮用3%果糖水导致胰岛素抵抗小鼠,再以小剂量链脲佐菌素(30mg/kg)腹腔注射1次,2周后诱导建立2型糖尿病小鼠模型。对照组动物则给予正常饲料及饮用水进行喂养。模型建立成功后,对模型2组大鼠进行胰岛素治疗,分别采用OPG和RANKLelisa试剂盒对正常动物模型和糖尿病动物模型血清和骨组织中OPG,RANKL含量进行比较分析,采用血糖分析仪对不同组动物的血糖进行比较分析,采用骨密度分析仪对动物的骨密度进行分析,了解高血糖对于骨密度及血清,骨组织中OPG,RANKL含量的影响以及胰岛素对高血糖骨质疏松造成的结果的影响。结果:相较于正常组大鼠,模型组大鼠血清及髂骨中OPG、血糖、糖化血红蛋白、髂骨密度表达显著下调(P0.05),而RANKL表达显著上调(P0.05),胰岛素处理的模型大鼠血清及骨中OPG含量较模型组大鼠显著升高,血清及骨组织中RANKL表达显著下调(P0.05)。结论:胰岛素能够显著降低2型糖尿病骨质疏松大鼠血清及骨组织中RANKL的表达,显著上调OPG的表达。

Effect of Insulin on the Serum and Bone OPG and RANKL Expressions in Type 2 Diabetic Rats with Osteoporosis

ABSTRACT Objective: To explore the effect of insulin on the serum and bone OPG(osteoprotegerin) and RANKL(OPG receptor activator nuclear factor-kB) expressions in the type 2 diabetic rats with osteoporosis. Methods: A rat model of type 2 diabetes with osteo- porosis was established by high-fat diet and 4% fructose water for 4 weeks, and then the rats were treated with streptozocin. The expres- sion of serum and bone OPG and RANKL between control and model group were analyzed with ELISA, bone density was detected by bone mineral density analyzer and the level of blood glucose were analyzed by blood glucose meter. Results: The expression of OPG, blood glucose, glycosylated hemoglobin ,bone density of serum and bone in model group were significantly lower than those control group(P<0.05), while the expression of RANKL was significantly higher than that of the control group (P<0.05), and insulin can increase the expression of OPG in serum and bone of model group, and decrease the expression of RANKL in serum and bone in model group (P<0.05). Conclusion: Insulin can decrease the expression of RANKL and increase the expression of OPG in serum and bone in type 2diabetic rats with osteoporosis.