微囊化K562细胞生长周期及代谢特性的研究

以K562细胞为模型，分别进行微囊化和游离培养，运用流式细胞术考察两种培养体系下细胞周期和生长代谢变化；建立数学模型，模拟了两种培养体系下细胞的生长活性和代谢特性。实验发现：微囊化培养过程中的K562细胞处于DNA合成期（S期）的百分含量显著高于游离培养，并且细胞保持较高的增殖活性。模型计算表明，所建模型动力学参数能够很好地描述微囊化和游离两种培养体系下细胞的代谢情况；对细胞活性的理论计算表明，微囊化的细胞具有较高的增殖和代谢活性，同时细胞能够较长时间保持此活性；模型参数表明，两种培养体系下，葡萄糖对细胞生长的影响无显著差别 (k^Free_L≈k^APA_L)，乳酸对游离培养细胞的生长具有明显抑制作用，但对微囊化培养细胞抑制作用较小(kFreeL>≈kAPAL)。

Characteristics of Cell Cycle and Metabolism in Microencapsulated K562 Cell Culture

Human K562 leukemia cells were cultured under free and microencapsulated condition, respectively. The cell cycles in the two kinds of cultures were investigated by flow cytometry. Moreover, mathematical model was established to simulate the cell viability and metabolized characteristic in different cultures. It was found that the cell percent in S phase was higher and the cell viability was better when cultured in microcapsule than that in free culture. The results showed that the model successfully described the substrate consumption and product formation in microencapsulated culture as well as in suspension culture. Based on the model, it was indicated that not only there was a higher proliferation and metabolic activity but also the time of the high activity could keep longer in microencapsulated culture. The parameters of the model showed that there was no significant difference between the two kinds of cultures when the influence of the glucose on the cell viability was concerned (kA(free) approximately = kA (APA)) but lactate had a obvious suppression effect on cell viability in free culture, and neglectable suppression in microencapsulated culture (kL(free) > kL(APA)).