Metabolism of trans-ferulic acid by the white-rot fungus sporotrichum pulverulentum |
| |
| Authors: | Jugal K Gupta Sven G Hamp John A Buswell Karl-Erik Eriksson |
| |
| Institution: | (1) Chemistry Department, Swedish Forest Products Research Laboratory, Box 5604, S-11486 Stockholm, Sweden;(2) Present address: Department of Microbiology, Panjab University, 160014 Chandigarh, India;(3) Present address: Department of Biology, Paisley College of Technology, Paisley, Renfrewshire, Scotland |
| |
| Abstract: | Ferulic acid metabolism was studied in wild-type Sporotrichum pulverulentum and its phenoloxidase-less mutant, Phe 3. High levels of reduced products which included coniferyl aldehyde, dihydroferulic acid and dihydroconiferyl alcohol were detected in culture filtrates. Small amounts of vanillic acid and methoxyhydroquinone were also found. In addition, products which possessed a methylated p-hydroxyl group were identified by mass spectrometry. The phenoloxidase-less mutant gave essentially the same reduced products as the wildtype. These persisted for longer periods in the culture medium. Three fungi known to produce large amounts of phenoloxidases exhibited a markedly different pattern of ferulic acid depletion.Abbreviations BSTFA
N,O-bis-(Trimethylsilyl)trifluoroacetamide
- Phe 3
phenoloxidase-less mutant |
| |
| Keywords: | Ferulic acid metabolism White-rot fungi Sporotrichum pulverulentum Reduction products 14CO2 evolution Phenol oxidase-less mutant |
| 本文献已被 SpringerLink 等数据库收录！ |
|
