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A quick method for purifying bile salt-activated lipases |
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| Authors: | Andrew N J Moore Helen Vakos Tracey A M Neville Peter Tonge Prabhat Arya Graham W Burton |
| Institution: | (1) Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, K1A 0R6 Ottawa, Ontario, Canada;(2) Department of Chemistry, University of Ottawa, 10 Marie Curie, K1N 6N5 Ottawa, Ontario, Canada |
| Abstract: | Summary A simple, one step chromatography procedure of general utility for purifying bile salt-activated lipases (BAL; cholesterol esterases) using a cholate affinity column is described. A key feature of the method is the use of a taurocholate concentration gradient in the eluent. The method has been used successfully to purify bovine and porcine BAL from commercial crude sources and BAL from human milk whey. |
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