Location of the extrinsic subunit PsbP in photosystem II studied by pulsed electron-electron double resonance |
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| Authors: | Mizue Asada Taishi Nishimura Kentaro Ifuku Hiroyuki Mino |
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| Affiliation: | 1. Division of Material Science (Physics), Graduate school of Science, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8602, Japan;2. Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606–8502, Japan |
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| Abstract: | ![]()
The binding site of the extrinsic protein PsbP in plant photosystem II was mapped by pulsed electron-electron double resonance, using mutant spinach PsbP (Pro20Cys, Ser82Cys, Ala111Cys, and Ala186Cys) labeled with 4-maleimido-TEMPO (MSL) spin label. The distances between the spin label and the Tyr160 neutral radical (YD 4RTI) to obtain the coordinates of YD4UB6), by rotating on Euler angles centered at fixed YD | |
| Keywords: | EPR electron paramagnetic resonance PELDOR pulsed electron-electron double resonance ESE electron spin echo CW continuous wave MW microwave Mes 2-morpholinoethasulfonic acid PS II photosystem II OEC oxygen-evolving complex D Tyr161 for higher plants (Tyr160 for cyanobacteria) of the D2 subunit in PS II MSL 4-maleimido-TEMPO TEMPO 2,2,6,6-tetramethylpiperidine 1-oxyl EM electron microscopy Photosystem II Extrinsic subunit PsbP EPR PELDOR |
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