The complete nucleotide sequence of the gene coding for botulinum type C1 toxin in the C-ST phage genome |
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| Authors: | K Kimura N Fujii K Tsuzuki T Murakami T Indoh N Yokosawa K Takeshi B Syuto K Oguma |
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| Institution: | Department of Epidemiology, Hokkaido Institute of Public Health, Hokkaido University, Japan. |
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| Abstract: | Two DNA fragments, 3 kbp and 7.8kbp, which encode the type C1 botulinum neurotoxin gene, were obtained from toxigenic bacteriophage DNA by treatment with a restriction enzyme. They were cloned into the plasmid vectors for nucleotide sequence determination. The nucleotide sequence contained a single open reading frame coding for 1,291 amino acids corresponding to a polypeptide with a molecular weight of 149,000. The amino acid sequence of the C1 toxin has a few regions highly homologous with tetanus toxin. |
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