Fine structural and immunohistochemical studies of goat adenohypophysial cells |
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Authors: | Dr Nobuyuki Shirasawa Hirotaka Kihara Fujio Yoshimura |
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Institution: | (1) Department of Anatomy, Jikei University School of Medicine, Tokyo, Japan;(2) Department of Anatomy, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, 105 Tokyo, Japan |
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Abstract: | Summary The fine structure of each type of anterior pituitary cell in the male goat was studied through the application of a superimposition technique in which adjacent thick sections were used to identify individual cells beforehand by light-microscopic immunohistochemistry. A cone of the pars intermedia protrudes into the pars anterior, being surrounded by the narrow pituitary cleft; the immunohistochemical appearances of the cells forming the cone resemble those of the pars anterior. Several follicles appear in the pars anterior. Ultrastructurally GH cells resemble prolactin cells. The secretory granules of both types are spherical; the diameter of the former is about 340 nm, whereas that of the latter is about 440 nm. ACTH cells are polygonal in shape with secretory granules, about 180 nm in diameter, scattered throughout the cytoplasm. TSH cells, which are spherical in shape, contain the smallest secretory granules, 150 nm in diameter. The highly electron-dense LH cells contain numerous secretory granules about 210 nm in diameter. Their nuclei are irregular with incisures. Thus, the anterior pituitary cells of the goat are ultrastructurally characteristic and species-specific. |
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Keywords: | Pituitary gland Fine structure Pituitary cell Immunocytochemistry Goat |
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