1H-NMR studies on nucleotide binding to the catalytic sites of bovine mitochondrial F1-ATPase |
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| Authors: | J Garin P V Vignais A M Gronenborn G M Clore Z Gao E Baeuerlein |
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| Affiliation: | Département de Recherche Fondamentale, Centre d'Etudes Nucleaires, Grenoble, France. |
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| Abstract: | The conformation of adenine nucleotides bound to bovine mitochondrial F1-ATPase was investigated using transfer nuclear Overhauser enhancement measurements. It is shown that all nucleotides investigated adopt a predominantly anti conformation when bound to the catalytic sites. Furthermore, the experiment suggests that 8-azido-ADP and 8-azido-ATP, which are predominantly in the syn conformation in solution, are in the anti conformation when bound to F1 catalytic sites. |
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