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| Roscovitine对C型尼曼-皮克病小鼠球状神经轴突形成的影响 | |
| 引用本文： | 张旻,赵娟,丁志刚,卜碧涛,王伟,Inez Vincent.Roscovitine对C型尼曼-皮克病小鼠球状神经轴突形成的影响[J].中国组织化学与细胞化学杂志,2007,16(1):1-5. |
| 作者姓名： | 张旻 赵娟 丁志刚 卜碧涛 王伟 Inez Vincent |
| 作者单位： | 1. 华中科技大学同济医学院附属同济医院神经内科,武汉,430030 2. 湖北省潜江市江汉油田中心医院神经内科,433124 3. Department of Pathology, University of Washington, Seattle, Washington, USA 98195 |
| 基金项目： | 国家自然科学基金;国家自然科学基金 |
| 摘 要： | 目的探讨细胞周期依赖性蛋白激酶抑制剂roscovitine对C型尼曼-皮克病(NPC)小鼠(npc-/-)球状神经轴突形成的影响。方法采用不同浓度的roscovitine(72,144,300 and 600nmoles/day)对5.5周龄的npc-/-小鼠进行两周侧脑室灌注,以DMSO灌注的同龄npc-/-小鼠为对照。抗非磷酸化神经细丝蛋白抗体SMI32和抗有丝分裂期磷酸化表位抗体MPM-2免疫染色检测小鼠脑内球状神经轴突的数量,Western blot检测细胞骨架蛋白磷酸化程度的变化。结果Roscovitine组球状神经轴突数量较DMSO组显著减少,神经细丝和tau蛋白的过度磷酸化程度较对照组明显下降(P<0.05)。结论细胞周期依赖性蛋白激酶抑制剂能明显改善NPC的神经元细胞骨架损害。 |
| 关 键 词： | 细胞周期依赖性蛋白激酶 C型尼曼-皮克病 球状神经轴突 |
| 文章编号： | 24307260 |
| 修稿时间： | 05 18 2006 12:00AM |
THE EFFECT OF ROSCOVITINE ON SPHEROID FORMATION IN MICE WITH NIEMANN-PICK DISEASE TYPE C |
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| Zhang Min,Zhaojuan,Ding Zhigang,Bu Bitao,Wang Wei,Inez Vincent.THE EFFECT OF ROSCOVITINE ON SPHEROID FORMATION IN MICE WITH NIEMANN-PICK DISEASE TYPE C[J].Chinese Journal of Histochemistry and Cytochemistry,2007,16(1):1-5. | |
| Authors: | Zhang Min Zhaojuan Ding Zhigang Bu Bitao Wang Wei Inez Vincent |
| Institution: | Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 1. Department of Neurology, Central Hospital, Jianghan Oil fieM , 2.Department of Pathology, University of Washington, Seattle, WA 98195, USA |
| Abstract: | Objective To investigate the effect of cyclin dependent kinase inhibitor roscovitine on spheroid formation in Niemann-Pick disease type C (NPC) mice.Methods Differently concentrated roscovitine (72,144,300 and 600nmoles/day) was infused into 5.5 week-old NPC-/-mice intracerebroventricularly for a 2-week period, using DMSO infused and same aged NPC-/-mice as controls. Anti-dephosphorylated neurofilament antibody SMI32 and anti-mitotic epitopes antibody MPM-2 were used to count the number of spheroids in different regions of mouse brain by immunohistochemical staining. Western blot was adopted to detect the phosphorylation status of cytoskeletal proteins. Results Compared with DMSO, roscovitine decreased the number of spheroids, attenuated the hyperphosphorylation of neurofilament and tau in brains of NPC mice significantly(P<0.05). Conclusion The cyclin dependent kinase inhibitor can improve the neuronal cytoskeletal lesion in NPC. |
| Keywords: | Roscovitine |
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