Effects of running training on in vitro brown adipose tissue thermogenesis in rats |
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Authors: | Tsukasa Nozu Kazue Kikuchi Koji Ogawa Akihiro Kuroshima |
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Institution: | (1) Laboratory of Physical Education, Hokkaido Tokai University, 078 Asahikawa, Japan;(2) Department of Physiology, Asahikawa Medical College, 078 Nishikagura, Asahikawa, Japan |
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Abstract: | Brown adipose tissue (BAT) is a major site of nonshivering thermogenesis (NST) during cold acclimation for most mammals. Repetitive nonthermal stress such as immobilization has been shown to enhance the capacity of NST as cold acclimation. In the present study, the effects of running training, another type of nonthermal stress, were investigated on in vitro thermogenesis and the cellularity of interscapular BAT in rats. The rats were subjected to treadmill running for 30 min daily at 30 m/min under 8° inclination for 4–5 weeks. In vitro thermogenesis was then measured in minced tissue blocks incubated in a Krebs-Ringer phosphate buffer containing glucose and albumin at 37° C, using a Clark type oxygen electrode. The trained rats showed less body weight gain during the experiment. The weights of BAT and epididymal white adipose tissue were smaller in the trained rats. Noradrenaline- and glucagon-stimulated oxygen consumption were also significantly smaller in the trained rats. The tissue DNA level was greater in the trained rats, but the DNA content per tissue pad did not significantly differ. The results indicate that running training reduces BAT thermogenesis, possibly as an adaptation to conserve energy substrates for physical work. |
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Keywords: | Thermogenesis Running training Exercise Brown adipose tissue |
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