Nuclear localization of Rad52 is pre-requisite for its sumoylation |
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| Authors: | Ohuchi Takashi Seki Masayuki Enomoto Takemi |
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| Institution: | a Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai 980-8578, Japan
b Tohoku University 21st Century COE Program “Comprehensive Research and Education Center for Planning of Drug development and Clinical Evaluation”, Sendai, Miyagi 980-8578, Japan |
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| Abstract: | In Saccharomyces cerevisiae, Rad52 plays major roles in several types of homologous recombination. Here, we found that rad52-K200R mutation greatly reduced sumoylation of Rad52. The rad52-K200R mutant exhibited defects in various types of recombination, such as intrachromosomal recombination and mating-type switching. The K200 residue of Rad52 is part of the nuclear localization signal (NLS), which is important for transport into the nucleus. Indeed, the addition of a SV40 NLS to Rad52-K200R suppressed the sumoylation defect of Rad52-K200R. These findings indicate that nuclear localization of Rad52 is pre-requisite for its sumoylation. |
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| Keywords: | Homologous recombination Mating-switching Sister chromatid recombination Rad52 Rad52- K200R Rad52-3KR SUMO Ubc9 NLS S cerevisiae |
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