Water fluxes through aquaporin-9 prime epithelial cells for rapid wound healing |
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| Authors: | Thommie Karlsson B Christoffer Lagerholm Elena Vikström Vesa M Loitto Karl-Eric Magnusson |
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| Institution: | 1. Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, SE-58185 Linköping, Sweden;2. Department of Physics, Chemistry and Pharmacy, MEMPHYS – Center for Biomembrane Physics & Danish Molecular Biomedical Imaging Center, University of Southern Denmark, CampusVej 55, DK-5230 Odense, Denmark;1. Institute for Bioengineering of Catalonia (IBEC), Cellular Biotechnology, Baldiri Reixac 15-21, 08028 Barcelona, Spain;2. Microsystems Laboratory (LMIS4), École Polytechnique Fédérale de Lausanne, Switzerland;3. Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain;1. Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;2. Department of Clinical Oncology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;3. Gastrointestinal Institute, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;4. Shanghai Biochip Company Limited, Shanghai, China;5. Department of Pathology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;1. Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, India;2. Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India |
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| Abstract: | Cells move along surfaces both as single cells and multi-cellular units. Recent research points toward pivotal roles for water flux through aquaporins (AQPs) in single cell migration. Their expression is known to facilitate this process by promoting rapid shape changes. However, little is known about the impact on migrating epithelial sheets during wound healing and epithelial renewal. Here, we investigate and compare the effects of AQP9 on single cell and epithelial sheet migration. To achieve this, MDCK-1 cells stably expressing AQP9 were subjected to migration assessment. We found that AQP9 facilitated cell locomotion at both the single and multi-cellular level. Furthermore, we identified major differences in the monolayer integrity and cell size upon expression of AQP9 during epithelial sheet migration, indicating a rapid volume-regulatory mechanism. We suggest a novel mechanism for epithelial wound healing based on AQP-induced swelling and expansion of the monolayer. |
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