Transduced Tat-glyoxalase protein attenuates streptozotocin-induced diabetes in a mouse model |
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| Authors: | Mi Jin Kim Dae Won Kim Byung Ryong Lee Min Jea Shin Young Nam Kim Seon Ae Eom Byung-Jae Park Yoon Shin Cho Kyu Hyung Han Jinseu Park Hyun Sook Hwang Won Sik Eum Soo Young Choi |
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| Affiliation: | 1. School of Molecular Bioscience, University of Sydney, NSW 2006, Australia;2. Woolcock Institute of Medical Research, NSW 2050, Australia;3. Discipline of Pharmacology, Bosch Institute, University of Sydney, NSW 2006, Australia;1. Department of Internal Medicine III, Division of Endocrinology and Metabolism, Medical University of Vienna, Vienna, Austria;2. Metabolic Unit, CNR Institute of Neuroscience, Padova, Italy;3. Department of Internal Medicine III, Division of Gastroenterology and Hepatology, Medical University of Vienna, Vienna, Austria;4. Department of Surgery, Division of Transplantation, Medical University of Vienna, Vienna, Austria;5. Unit of Gendermedicine, Medical University of Vienna, Vienna, Austria. |
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| Abstract: | Diabetes mellitus (DM) is characterized by hyperglycemia. Glyoxalase 1 (GLO) has considerable potential as a possible therapeutic agent for DM. However, the precise action of GLO remains unclear in DM. In this study, we examined the protective effects of GLO protein in a streptozotocin (STZ)-induced diabetes animal model using cell-permeable Tat-GLO protein. Purified Tat-GLO protein was efficiently transduced into RINm5F cells in a time- and dose-dependent manner and protected cells against sodium nitroprusside (SNP)-induced cell death and DNA fragmentation. Furthermore, Tat-GLO protein significantly inhibited blood glucose levels and altered the serum biochemical parameters in STZ-induced diabetic mice. These results demonstrate that transduced Tat-GLO protein protects pancreatic cells by the inhibition of STZ-mediated toxicity. Therefore, Tat-GLO protein could be useful as a therapeutic agent against DM. |
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