Somatic embryogenesis from ovaries,developing ovules and immature zygotic embryos,and improved embryo development of <Emphasis Type="Italic">Castanea sativa</Emphasis> |
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Authors: | U?Sauer Email author" target="_blank">E?WilhelmEmail author |
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Institution: | (1) Austrian Research Centre Seibersdorf, A-2444 Seibersdorf, Austria |
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Abstract: | Somatic embryogenesis of European chestnut (Castanea sativa Mill.) was obtained using juvenile tissue cultured on P24 medium with 5 M 2,4-dichlorophenoxyacetic acid plus 0.5 M 6-benzylaminopurine (BA) for three weeks and then cultured on 0.89 M BA. Induction frequency with ovaries ranged from 2.0 to 19.1 % and was observed in tissue collected 2 to 8 weeks postanthesis, ovules used as a starting tissue gained 0.8 to 7.8 %, 3 to 9 weeks postanthesis. Zygotic embryos collected 5 to 10 weeks postanthesis formed 10.5 to 57.1 % somatic embryos, respectively. The culture lines were maintained via secondary embryogenesis on P24 medium with 0.89 M BA. Development and maturation were stimulated on P24 medium with increased agar concentration (1.1 %). Five plantlets were transferred to substrate and acclimatized successfully in greenhouse. |
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Keywords: | 6-benzylaminopurine 2 4-dichlorophenoxyacetic acid European chestnut Fagaceae |
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