Translational efficiency of mRNA in yeast cells is not increased by plant viral leader sequences |
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| Authors: | Joop J. van der Heuvel Hendrik A. Raué |
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| Affiliation: | (1) Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Vrije Universiteit, de Boelelaan 1083, NL-1081 HV Amsterdam, The Netherlands;(2) Present address: Gesellschaft für Biotechnologische Forschung (GBF), Mascheroder Weg 1, 3300 Braunschweig, Federal Republic of Germany |
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| Abstract: | Summary Synthetic oligonucleotides encoding the 5 -non-translated (leader) sequence of the coat protein mRNA of alfalfa mosaic virus RNA 4 or the  leader sequence of tobacco mosaic virus RNA were used to replace the natural leader region of the yeast phosphoglycerate kinase (PGK1) mRNAs and the translational efficiency of the chimeric mRNA was determined in yeast cells. In neither case did we observed a significant increase compared to the translational efficiency shown by the wild-type PGK mRNA, in contrast to the known stimulatory effect of these leader sequences on translation in mammalian, plant and bacterial in-vivo and/or in-vitro systems. The same result was obtained when the translational efficiencies in yeast cells of Escherichia coli  -galactosidase mRNAs carrying the PGK or either of the two viral leader sequences were compared.Offprint requests to: H. A. Raué |
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