Strains of Peanut Stripe Potyvirus Rapidly Identified by Profiling Peptides of the Virion Proteins |
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Authors: | Kruapan KittipakornNeil M McKern Adrian J Gibbs Dharma D Shukla |
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Institution: | CSIRO, Division of Biomolecular Engineering, 343 Royal Parade, Parkville, Victoria 3052, Australia. |
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Abstract: | Virion proteins of five potyvirus isolates from groundnut in Thailand which induce a range of symptoms were compared with those from the type strains of peanut stripe potyvirus (PStV) and peanut mottle potyvirus (PeMoV). Profiles obtained by high performance liquid chromatography (HPLC) of tryptic peptide digests of the virion proteins of all five Thai isolates and PStV were very similar, whereas that of PeMoV was quite different. The results indicate that the Thai isolates are strains of PStV, and reaffirm the value of HPLC peptide profiling for rapidly identifying potyviruses. |
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