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Immunocytochemical localization of CRF in the ovine hypothalamus |
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| Authors: | W.K. Paull Jan Schöler Akira Arimura Chester A. Meyers J.K. Chang D. Chang M. Shimizu |
| Affiliation: | 1. Department of Anatomy, Tulane University School of Medicine, New Orleans, LA 70112, USA;2. Department of Anatomy, Louisiana State University School of Medicine, New Orleans, LA 70112, USA;3. Department of Medicine, Tulane School of Medicine, New Orleans, LA 70112, USA;4. Department of Anatomy, Tulane School of Medicine, New Orleans, LA 70112, USA;1. Laboratories for Molecular Neuroendocrinology and Diabetes Tulane University Hebert Research Center, Belle Chasse, LA 70037, USA;5. National Institute of Health, Bethesda, MD, USA;6. Peninsula Laboratories, San Carlos, CA, USA |
| Abstract: | A population of neuronal cell bodies and their fiber pathways have been elucidated within the ovine hypothalamus. The immunoreactive neurons were located in the anterior and dorsal hypothalamus interspersed throughout the paraventricular nucleus. These perikarya were only observed when an antiserum that was generated against the C-terminal of CRF was employed. A dense fiber projection traversed the medial-basal hypothalamus and ended within the palisade-contact zone of the median eminence and neural stem. Fibers were revealed by antisera generated against both the N-terminal and the C-terminal of CRF. Antisera pre-absorbed with synthetic CRF failed to yield immunoreactivity. |
| Keywords: | CRF Immunocytochemistry Hypothalamus |
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