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The influence of maltoporin affinity on the transport of maltose and maltohexaose into Escherichia coli
Affiliation:Department of Microbiology, University of Sydney, Sydney, NSW 2006 Australia;Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen 9747 AG, the Netherlands
Abstract:
The kinetics of maltose transport and its inhibition by maltohexaose were investigated using Escherichia coli strains with engineered modifications of maltoporin. The permeation of lactose through maltoporin was also measured, as well as its inhibition by maltohexaose. Based on these results, the role of the maltoporin binding site in transport was evaluated.
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