首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Bub3p Facilitates Spindle Checkpoint Silencing in Fission Yeast
Authors:Vincent Vanoosthuyse  John C Meadows  Sjaak JA van der Sar  Jonathan BA Millar  Kevin G Hardwick
Institution:*Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, EH9 3JR, United Kingdom; ;Department of Biological Science, University of Warwick, Coventry, CV4 7AL, United Kingdom
Abstract:Although critical for spindle checkpoint signaling, the role kinetochores play in anaphase promoting complex (APC) inhibition remains unclear. Here we show that spindle checkpoint proteins are severely depleted from unattached kinetochores in fission yeast cells lacking Bub3p. Surprisingly, a robust mitotic arrest is maintained in the majority of bub3Δ cells, yet they die, suggesting that Bub3p is essential for successful checkpoint recovery. During recovery, two defects are observed: (1) cells mis-segregate chromosomes and (2) anaphase onset is significantly delayed. We show that Bub3p is required to activate the APC upon inhibition of Aurora kinase activity in checkpoint-arrested cells, suggesting that Bub3p is required for efficient checkpoint silencing downstream of Aurora kinase. Together, these results suggest that spindle checkpoint signals can be amplified in the nucleoplasm, yet kinetochore localization of spindle checkpoint components is required for proper recovery from a spindle checkpoint-dependent arrest.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号