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Studies of sub-human primate (marmoset) pluripotent hemopoietic stem cells (CFU-GEMM) in vitro |
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| Authors: | R Michalevicz C M Hetherington I Hann H A Blacklock |
| Abstract: | Pluripotent hemopoietic progenitor cells (CFU-GEMM) grow in vitro from marmoset bone marrow using a modified human CFU-GEMM assay. Characteristics of growth are similar to those reported in the human CFU-GEMM assay. The number of CFU-GEMM/10(5) marrow cells from marmoset bone marrow is approximately four times that grown in human marrow in our laboratory. Data concerning EPO and other requirements for growth of CFU-GEMM demonstrate an assay for the pluripotent hemopoietic progenitor in the marmoset. This assay may be useful in designing preclinical primate bone marrow transplant experiments. |
| Keywords: | Marmoset Mixed colony CFU-GEMM BFU-E (burst-forming unit erythroid) CFU-GM (colony-forming unit granulocytic macrophage) CFU-MK (colony-forming unit megakaryocytic) |