Regeneration of haploid plants from isolated microspores of asparagus (Asparagus officinalis L.) |
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Authors: | Cun-Jin Zhang Hong-Long Wang Yun Ma Yu-Qing Kang |
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Institution: | (1) Department of Biology, Tianjin Institute of Education, 300020 Tianjin, China;(2) Department of Biology, Tianjin Normal University, 300074 Tianjin, China;(3) Present address: INRA, Symbiotes des Racines, 34060 Montpellier, France |
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Abstract: | High percentages of micro-calli and micro-derived embryos were produced from isolated asparagus microspores at late uninucleate stage on MS liquid medium supplemented with 1.0 mg l–1 2,4-D and 0.5 mg l–1 BA. Two types of calli, namely compact callus (CC) and loose callus (LC), were found. Plantlets were regenerated via organogenesis, when these calli were transferred onto MS solid medium supplemented with 1.0 mg l–1 BA and 0.2 mg l–1 IBA 6 weeks. Embryos were produced from liquid cultured microspores, or from solid cultured micro-calli. The frequencies of haploid plant production from organogenesis and embryogenesis were compared. Effects of plant growth regulators on callus production, plantlet regeneration, and haploid plant production were tested. The combination of BA 1.0 mg l–1 and IBA 0.2 mg l–1 resulted the highest precentage of haploid plant production (7.7% from CC, 4.3% from LC).Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- IBA
3-indolybutyric acid
- BA
6-binzyladinine
- NAA
naphtalene acetic acid
- MS
Murashige and Skoog |
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Keywords: | Asparagus officinalis L Microspore culture Embryogenesis Haploid Plant growth regulators |
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