Transdifferentiation of mucous neck cells into chief cells in fundic gastric glands shown by GNA lectin histochemistry |
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Authors: | Laura Gómez-Santos Edurne Alonso Lucio Díaz-Flores Juan Francisco Madrid Francisco José Sáez |
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Institution: | 1. Department of Cell Biology and Histology, Training and Research Unit: Reproduction, Development, Aging and Cancer (TRU/UFI 11/44), University of the Basque Country UPV/EHU, Leioa, Vizcaya, Spain;2. Department of Anatomy, Pathology, Histology and Radiology, University of La Laguna, Tenerife, Spain;3. Department of Cell Biology and Histology, Regional Campus of International Excellence “Campus Mare Nostrum”, IMIB-Arrixaca, University of Murcia, Espinardo, Murcia, Spain |
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Abstract: | The epithelium of the gastric mucosa and its glands in the corpus of rat stomach contains mucous surface cells (MSCs), parietal cells, mucous neck cells (MNCs), zymogenic or chief cells (ZCs), several types of enteroendocrine cells, and intermediate cells with characteristics between MNCs and ZCs also called transitional or prezymogenic cells (pre-ZCs).The aim of our work was to analyze the expression of Mannose (Man) in the rat gastric glands by means of Galanthus nivalis lectin (GNA) histochemistry to identify the differences between MNC, pre-ZCs and ZCs and to establish the relationships between these cells. Most of the cytoplasm of MNCs was negative for GNA histochemistry. Intensity of GNA labeling in the gastric gland showed a graduation from pre-ZCs (weak labeling) to ZCs (moderate labeling). Labeling of ZCs was stronger at the perinuclear and apical cytoplasm.In the last years, strong evidence has been reported supporting that ZCs differentiate from MNCs. Our work also supports the origin of ZCs from MNCs, because the GNA labeling graduation might be due to oligosaccharides which are not expressed in MNCs, start to express in pre-ZCs and are more abundant in ZCs, indicating that differentiation from MNCs to ZCs is a process in which glycans with Man moieties are synthesized. |
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Keywords: | AAL ABC avidin-biotin-peroxidase complex BSA bovine serum albumine DAB diaminobencidine GNA HPA Man Mannose MNC mucous neck cell MSC mucous surface cell NeuAc sialid acid PBS phosphate buffered saline PNA peanut agglutinin PNGase-F Pre-ZC pre-zymogenic cell SSC somatic stem cell TBS Tris buffered saline ZC zymogenic cell Glandular stomach Oligosaccharides Lectin Differentiation Mannose |
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