Events in the amoebal-plasmodial transition ofPhysarum polycephalum studied by enrichment for committed cells |
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Authors: | A B Blindt Anne M Chainey Jennifer Dee K Gull |
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Institution: | (1) Department of Genetics, University of Leicester, University Road, LEI 7RH Leicester, UK;(2) Biological Laboratory, University of Kent, Canterbury, Kent |
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Abstract: | Summary Amoebae of strain CLof Physarum polycephalum undergo apogamic development to form multinucleate plasmodia. During the amoebalplasmodial transition, large uninucleate cells become irreversibly committed to plasmodium development. In developing cultures, amoebae lose the ability to flagellate before they become committed. Enriched suspensions of committed cells can be obtained by inducing asynchronous differentiating cultures to flagellate and passing the cells through a glass bead column. Committed cells can be cultured to form plasmodia on bacterial lawns or in axenic liquid medium but cannot be cultured on axenic agar medium. Uninucleate committed cells express tubulin isotypes characteristic of amoebae, but after culture in axenic liquid medium, the cells express plasmodial specific tubulin isotypes.Abbrevations SDM
Semi-defined medium
- DSDM
Dilute semidefined medium
- LIA
Liver infusion agar
- SBS
Standard bacterial suspension
- IEF
Isoelectric focussing
- SDS
Sodium dodecyl sulphate
- PAUF
Precommitted amoebae unable to flagellate (for the explanation of these cells see text). |
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Keywords: | Physarum Commitment Development Tubulin Flagellation |
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