| 2,4 -D对甘薯体细胞胚胎发生的调控 |
| |
| 引用本文： | 崔红,丁群星,桂耀林,郭仲琛.2,4 -D对甘薯体细胞胚胎发生的调控[J].植物学报,1999,16(4):411-415. |
| |
| 作者姓名： | 崔红 丁群星 桂耀林 郭仲琛 |
| |
| 作者单位： | 1(厦门大学生物系 厦门 361005） 2(中国科学院植物研究所 北京 100093） |
| |
| 摘 要： | 将来源于‘徐薯18’叶片的胚性愈伤组织，接种在含有不同2,4-D浓度的液体MS培养基中进行悬浮培养，悬浮细胞表现出不同的形态结构、分裂方式和发育途径：2,4-D浓度为1 mg/L时，细胞均等分裂，增殖迅速；不含2,4-D时，细胞多进行不均等分裂，并发育成体细胞胚。不同2,4-D浓度中培养的悬浮细胞，其胞外过氧化物同工酶谱及其随时间变化的方式有很大差异，并与细胞的生长、发育过程密切相关。
|
| 关 键 词： | 甘薯 体胚发生 悬浮培养 过氧化物同工酶 |
| 收稿时间： | 1998-04-13 |
| 修稿时间： | 1998-07-06 |
2,4-D Regulated Somatic Em bryogenesis of Sweet Potato |
| |
| CUI Hong,DING Qun-Xing,GUI Yao-Lin and GUO Zhong-Chen.2,4-D Regulated Somatic Em bryogenesis of Sweet Potato[J].Bulletin of Botany,1999,16(4):411-415. |
| |
| Authors: | CUI Hong DING Qun-Xing GUI Yao-Lin and GUO Zhong-Chen |
| |
| Institution: | 1(Department of Biology, Xiamen University, Xiamen 361005) 2(Institute of Botany, The Chinese Academy Sciences, Beijing 100093) |
| |
| Abstract: | Divergences were discovered in cell morphology, mode of cell division and passage of cell differentiation, when embryogenic calli induced from leaves of ‘Xushu 18’ sweet potato were suspension-cultured in modified MS medium with different concentrations of 2, 4-D. Cells grew
quickly and divided equally in medium containing 1 mg/L 2,4-D. When 2,4-D was deleted, the cells divided unequally and developed into embryos. Extracellular pemxidase isozyme patterns changed greatly during suspension culture with different concentrations of 2,4-D and were closely related to cell development process. |
| |
| Keywords: | , |
|
| 点击此处可从《植物学报》浏览原始摘要信息 |
| 点击此处可从《植物学报》下载免费的PDF全文 |
|
