花蓟马雄虫释放的聚集信息素的分离和鉴定

明确花蓟马Frankliniella intonsa雄虫释放的聚集信息素对于花蓟马生物防治具有重要意义。但目前除了西花蓟马F. occidentalis， 关于其他蓟马雄虫释放的聚集信息素及其成分鉴定的研究未有报道。本研究采用Y型嗅觉仪测定花蓟马雌雄成虫和西花蓟马雌雄成虫对花蓟马雄虫和西花蓟马雄虫气味源的嗅觉反应； 用固相微萃取（solid-phase microextraction,SPME）方法提取花蓟马雄虫挥发物， 利用含有BGB-176 SE或CP-chirasil-Dex CB 2种不同手性柱的气-质联用仪（gas chromatography-mass spectrometry, GC-MS）对挥发物进行分离和鉴定。行为学试验结果表明： 花蓟马雄虫气味源对其雄性及雌性成虫均具有明显的吸引作用。经GC-MS分析， 花蓟马雄虫挥发物中含有2种主要的化合物， 分别为(R)-lavandulyl acetate和neryl (S)-2-methylbutanoate， 其与已报道的西花蓟马雄虫释放的聚集信息素成分相同。但定量分析结果表明， (R)-lavandulyl acetate与neryl (S)-2-methylbutanoate在花蓟马和西花蓟马雄虫中释放的比例不同； 行为学试验进一步证明， 2种组分比例的不同在花蓟马和西花蓟马种间识别过程中发挥重要作用。本研究推测花蓟马属的昆虫释放的聚集信息素组分可能是相同的。

Isolation and identification of the aggregation pheromone released by male adults of Frankliniella intonsa(Thysanoptera:Thripidae)

Identification of aggregation pheromone released by Frankliniella intonsa males play a key role in bio-control of this pest.Except for the western flower thrips Frankliniella occidentalis,little is understood on aggregation pheromones in other thrips species.In the laboratory we tested the behavioral responses of adult F.intonsa and F.occidentalis to odor sources emitted from their corresponding adult males with Y-tube olfactometer.Head-space volatiles released by adult males of F.intonsa were collected using solid-phase microextraction(SPME) and identified by gas chromatography-mass spectrometry(GC-MS) with different GC columns(BGB-176 SE and CP-chirasil-Dex CB).Behavioral results showed that both male and female F.intonsa were attracted to the odors released by conspecific males.GC-MS analysis showed that two major components in head-space volatiles were released by F.intonsa,i.e.,(R)-lavandulyl acetate and neryl(S)-2-methylbutanoate,which are the same compounds that are reported in volatiles from F.occidentalis males.However,quantitative analysis showed that the ratios of(R)-lavandulyl acetate to neryl(S)-2-methylbutanoate released by F.intonsa and F.occidentalis adults differed.Behavioral experiments further showed that the difference in the ratio of the two components plays an important role in interspecies identification between F.intonsa and F.occidentalis.The results suggest that different species of thrips of the same genus Frankliniella may share the same aggregation pheromones.