| 基因枪法获得GAI转基因橡胶植株的研究 |
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| 引用本文： | 洪磊,王颖,陈雄庭,张秀娟.基因枪法获得GAI转基因橡胶植株的研究[J].热带亚热带植物学报,2010,18(2):165-169. |
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| 作者姓名： | 洪磊 王颖 陈雄庭 张秀娟 |
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| 作者单位： | 洪磊(中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101;庐江中学,安徽,庐江,231500);王颖,陈雄庭,张秀娟(中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101) |
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| 基金项目： | 国家自然科学基金，中国热带农业科学院中央级公益性科研院所基本科研业务费专项 |
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| 摘 要： | 将含有Camv35S启动子、卡那霉素抗性基因和GUS报告基因,目的基因为GAI基因的转化载体质粒pBI121,通过基因枪轰击巴西橡胶树(Hevea brasiliensis Muell-Arg.)花药愈伤组织,50 mg L~(-1)卡那霉素的继代培养基进行抗性筛选.获得了抗性再生植株,经过PCR、Southern检测结果表明:GAI基因已经成功转入橡胶树基因组中.
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| 关 键 词： | 巴西橡胶树 基因枪 GAI基因 |
| 收稿时间： | 2009/3/25 0:00:00 |
| 修稿时间： | 2009/11/30 0:00:00 |
Obtaining the GAI Transgenic Plants of Hevea brasiliensis by Particle Bombardment |
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| HONG Lei,WANG Ying,CHEN Xiong-ting and ZHANG Xiu-juan.Obtaining the GAI Transgenic Plants of Hevea brasiliensis by Particle Bombardment[J].Journal of Tropical and Subtropical Botany,2010,18(2):165-169. |
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| Authors: | HONG Lei WANG Ying CHEN Xiong-ting and ZHANG Xiu-juan |
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| Institution: | HONG Lei1,2,WANG Ying1,CHEN Xiong-ting1,ZHANG Xiu-juan1(1.Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China,2.Lujiang High School,Lujiang 231500,China) |
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| Abstract: | The vector pBI121 particle,which contains Camv35S promoter,kanamycin resistance gene,GUS reporter gene and GAI gene,was transfered into the Hevea brasiliensis anther callus by particle bombardment.The regenerated plantlets were obtained in the subculture media supplemented with kanamycin of 50 mg L-1.PCR and Southern Blotting demonstrated that the GAI gene was transferred into the H.brasiliensis genome successfully. |
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| Keywords: | Hevea brasiliensis Particle bombardment GAI gene |
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