| 白杨派树种亲缘关系的RAPD分析 |
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| 引用本文： | 傅明洋,樊军锋,周永学,高建社. 白杨派树种亲缘关系的RAPD分析[J]. 西北植物学报, 2009, 29(12) |
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| 作者姓名： | 傅明洋 樊军锋 周永学 高建社 |
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| 作者单位： | 西北农林科技大学,林学院,陕西杨陵,712100 |
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| 基金项目： | "十一五"国家科技支撑计划课题子专题毛白杨杂交育种与新品种示范，西北农林科技大学唐仲英育种基金专项 |
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| 摘 要： | 运用RAPD技术对白杨派中7个树种和3个人工杂交种共计50个无性系的亲缘关系进行分析.结果表明:筛选出的26条随机引物共扩增产生222条谱带,其中200条为多态性谱带,多态性带达90.09%,且种间遗传变异大于种内无性系间的遗传变异.聚类分析结果显示,参试的50个材料可分为2大类:第一类由山杨、响叶杨、河北杨组成;第二类由毛白杨、银毛杨、84K杨、银白杨、银灰杨、新疆杨和银新杨组成;3个人工杂交种与白杨亚派树种的亲缘关系较近;人工杂交种银毛杨与84K杨间亲缘关系最近.
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| 关 键 词： | 白杨派 亲缘关系 遗传距离 |
Relationship among Species of Sect.Populus Using RAPD Markers |
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| FU Ming-yang,FAN Jun-feng,ZHOU Yong-xue,GAO Jian-she. Relationship among Species of Sect.Populus Using RAPD Markers[J]. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(12) |
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| Authors: | FU Ming-yang FAN Jun-feng ZHOU Yong-xue GAO Jian-she |
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| Affiliation: | FU Ming-yang,FAN Jun-feng,ZHOU Yong-xue,GAO Jian-she(College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | The study analyzes the relationship of Sect.Populus by random amplified polymorphic DNA(RAPD) technique.7 species and 3 artificial hybrids were used in this experiment.Every species has 5 different clones except the 3 artificial hybrids.26 primers were selected for RAPD.Among the total of 222 bands,there are 200 bands(90.09%) were detected to be polymorphic.The genetic distances demonstrate that the diversity between all species is larger than that of the clones from every species.The phylogenetic dendrogra... |
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| Keywords: | RAPD Sect.Populus relationship genetic distance RAPD |
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