A specific endonuclease from Brevibacterium albidum |
| |
Authors: | Richard E Gelinas Phyllis A Myers Gary H Weiss Richard J Roberts Kenneth Murray |
| |
Institution: | Cold Spring Harbor Laboratory Cold Spring Harbor, N.Y. 11724, U.S.A.;University of Edinburgh Edinburgh EH9 3JR, Scotland |
| |
Abstract: | A new restriction-like endonuclease, BalI, has been partially purified from Brevibacterium albidum. This enzyme cleaves bacteriophage λ DNA at least 18 times and adenovirus-2 DNA at least 16 times, but does not cleave simian virus 40 DNA. All sites cleaved by BalI are also cut by the specific endonuclease HaeIII from Haemophilus aegyptius. The recognition sequence of BalI is 5′-T-G-G ↓ C-C-A-3′ 3′-A-C-C ↓ G-G-T-5′ and the cleavage site is indicated by the arrows. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|