Production and cytogenetic characterization of intertribal somatic hybrids between <Emphasis Type="Italic">Brassica napus</Emphasis> and <Emphasis Type="Italic">Isatis indigotica</Emphasis> and backcross progenies |
| |
Authors: | Xue-zhu Du Xian-hong Ge Xing-cheng Yao Zhi-gang Zhao Zai-yun Li |
| |
Institution: | (1) College of Life Science, Hubei University, 430062 Wuhan, People’s Republic of China;(2) National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement, College of Plant Science and Technology, Huazhong Agricultural University, 430070 Wuhan, People’s Republic of China;(3) Present address: Qinghai Academy of Agricultural and Forestry Sciences, Qinghai University, 810016 Xining, Qinghai, People’s Republic of China; |
| |
Abstract: | Intertribal somatic hybrids between Brassica napus (2n = 38, AACC) and a dye and medicinal plant Isatis indigotica (2n = 14, II) were obtained by fusions of mesophyll protoplasts. From a total of 237 calli, only one symmetric hybrid (S2) and
five asymmetric hybrids (As1, As4, As6, As7 and As12) were established in the field. These hybrids showed some morphological
variations and had very low pollen fertility. Hybrids S2 and As1 possessed 2n = 52 (AACCII), the sum of the parental chromosomes, and As12 had 2n = 66 (possibly AACCIIII). Hybrids As4, As6 and As7 were mixoploids (2n = 48–62). Genomic in situ hybridization analysis revealed that pollen mother cells at diakinesis of As1 contained 26 bivalents
comprising 19 from B. napus and 7 from I. indigotica and mainly showed the segregation 26:26 at anaphase I (AI) with 7 I. indigotica chromosomes in each polar group. Four BC1 plants from As1 after pollinated by B. napus resembled mainly B. napus in morphology but also exhibited some characteristics from I. indigotica. These plants produced some seeds on selfing or pollination by B. napus. They had 2n = 45 (AACCI) and underwent pairing among the I. indigotica chromosomes and/or between the chromosomes of two parents at diakinesis. All hybrids mainly had the AFLP banding patterns
from the addition of two parents plus some alterations. B. napus contributed chloroplast genomes in majority of the hybrids but some also had from I. indigotica. Production of B. napus–I. indigotica additions would be of considerable importance for genome analysis and breeding. |
| |
Keywords: | Brassica napus Isatis indigotica Intertribal hybrids Somatic hybridization Chloroplast genome |
本文献已被 SpringerLink 等数据库收录! |
|