Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway |
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| Institution: | 1. Key Laboratory of Industrial Biocatalysis (Ministry of Education), Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China;2. Tsinghua Innovation Center in Dongguan, Dongguan, 523808, China;3. Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China;4. School of Physiology, Pharmacology & Neuroscience, Faculty of Life Sciences, University of Bristol, Bristol, BS8 1TD, United Kingdom;1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China;2. Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing, 100048, China;3. State Key Laboratory of Transducer Technology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China;4. University of Chinese Academy of Sciences, Beijing, 100049, China;1. Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China;2. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China;3. Shandong Energy Institute, Qingdao, 266101, China;4. Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China;5. University of Chinese Academy of Sciences, Beijing, 100049, China;6. Marine Biology and Biotechnology Laboratory, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China;7. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China;1. BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK;2. Chemical Engineering Department, Universidad de La Frontera, Temuco, Chile;3. Scientific and Technological Bioresources Nucleus BIOREN-UFRO, Universidad de La Frontera, Temuco, Chile;4. Hub for Biotechnology in the Built Environment, Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Ellison Building, Newcastle upon Tyne, NE1 8ST, UK;5. School of Pharmacy, University Park, University of Nottingham, Nottingham, NG7 2RD, UK;1. Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China;2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China;3. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China;4. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing, 210014, China |
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| Abstract: | 1,5-Pentanediol (1,5-PDO) is a high value-added chemical which is widely used as a monomer in the polymer industry. There are no natural organisms that could directly produce 1,5-PDO from renewable carbon sources. In this study, we report metabolic engineering of Escherichia coli for high-level production of 1,5-PDO from glucose via a cadaverine-derived pathway. In the newly proposed pathway, cadaverine can be converted to 1,5-PDO via 5-hydroxyvalerate (5-HV) by introducing only one heterologous enzyme in E. coli. Different endogenous genes of E. coli were screened and heterologous carboxylic acid reductase genes were tested to build a functional pathway. Compared to the previously reported pathways, the engineered cadaverine-based pathway has a higher theoretical yield (0.70 mol/mol glucose) and higher catalytic efficiency. By further combining strategies of pathway engineering and process engineering, we constructed an engineered E. coli strain that could produce 2.62 g/L 1,5-PDO in shake-flask and 9.25 g/L 1,5-PDO with a yield of 0.28 mol/mol glucose in fed-batch fermentation. The proposed new pathway and engineering strategies reported here should be useful for developing biological routes to produce 1,5-PDO for real application. |
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| Keywords: | 1 5-Pentanediol Cadaverine Pathway engineering Metabolic engineering |
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