Raffinose in seedlings of winter vetch (<Emphasis Type="Italic">Vicia villosa</Emphasis> Roth.) under osmotic stress and followed by recovery |
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Authors: | Lesław B Lahuta Ryszard J Górecki |
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Institution: | (1) Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 1A/103A, 10-718 Olsztyn, Poland |
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Abstract: | During germination of winter vetch (Vicia villosa Roth.) seeds, the degradation of raffinose family oligosaccharides and galactosyl pinitols occurred faster in axis than in
cotyledons. After 7 days of germination, all α-d-galactosides disappeared and the soluble carbohydrates in seedling tissues consisted of d-pinitol, sucrose, fructose, glucose and myo-inositol. Osmotic stress caused by incubation of seedlings in PEG 8000 solution (−0.5, −1.0, and −1.5 MPa) for 48 h induced
the activity of crucial enzymes of the RFOs pathway, i.e. galactinol synthase and raffinose synthase, in both the root and
epicotyl but not in cotyledons. The root and epicotyl accumulated elevated amounts of galactinol and raffinose as the osmotic
potential was lowered. This process was transient because when PEG solution was replaced with water, galactinol and raffinose
were degraded, thus confirming their direct involvement in the response of tissues to osmotic stress. Among other soluble
carbohydrates, only sucrose accumulated in response to stress. The results did not show potential role of d-pinitol in the adjustment of winter vetch seedlings to osmotic stress. |
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