Preparation and application of anti-idiotypic antibody against anti-gibberellin A4 antibody. |
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| Authors: | Y Suzuki N Shimada R Niwa M Yokoi M Nakajima N Murofushi I Yamaguchi |
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| Institution: | Department of Applied Biological Chemistry, University of Tokyo, Japan. ayoshi@hongo.ecc.u-tokyo.ac.jp |
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| Abstract: | A monoclonal anti-idiotypic antibody was raised against anti-gibberellin A4 (GA4) antibody, which recognizes biologically active gibberellins such as GA1 and GA4 specifically. Amino acid sequences of variable regions of both anti-GA4 and anti-idiotypic antibodies were analyzed. By using the property of the anti-idiotypic antibody to compete with GA1/4 in binding to the anti-GA4 antibody, we successfully applied the anti-idiotypic antibody to ELISA as a tracer for measuring GA1/4. The single-chain Fv (scFv) gene of the anti-idiotypic antibody was constructed, and scFv expressed in E. coli showed binding activity to anti-GA4 antibody. These results suggest the possible application of anti-idiotypic antibody as a handy and stable source of an enzymatic tracer for ELISA by production of fusion protein of the scFv and an appropriate enzyme. |
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