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In vitro antioxidant and antitumor activities of polysaccharides extracted from Asparagus officinalis |
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| Authors: | Qingsheng Zhao Bingxian XieJun Yan Fangchun Zhao Jie Xiao Lingyun Yao Bing Zhao Yunxiang Huang |
| Affiliation: | a National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190 Beijing, PR China b Graduate University of Chinese Academy of Sciences, 100049 Beijing, PR China c Model Animal Research Center, MOE of Key Laboratory Model Animal for Disease Study, Nanjing University, Nanjing 210061, China d Zhejiang Provincial Key Lab for Technology and Application of Model Organisms, School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, China e Qinhuangdao Chang Sheng Agricultural Science and Technology Co., Ltd., Qinhuangdao 066006, PR China |
| Abstract: | Ultrasonic circulating extraction technology was applied for the polysaccharide extraction from Asparagus officinalis. The crude polysaccharides were deproteinized by Sevag method and three main polysaccharide fractions, AOP-4, AOP-6 and AOP-8 were obtained by fractional precipitation with gradient concentrations of ethanol (40%, 60% and 80%). The in vitro antitumor and antioxidant activities of the polysaccharide fractions were evaluated by MTT assay and free radical-scavenging assay, respectively. Deproteinized AOPs showed higher antioxidant and antitumor activities than crude AOP. AOP-4 with molecular weight 5.75 × 104 Da showed significant function of scavenging hydroxyl radical. Three AOP fractions had significant antitumor activity against HeLa and BEL-7404 cells in a dose dependent manner. Furthermore, the inhibit activity of AOP-4 against HeLa cells was higher than those of other AOPs and the inhibition rate reached 83.96% at the concentration of 10 mg/mL. These results indicated that the AOP might be useful for developing natural safe antitumor drugs or health food. |
| Keywords: | Asparagus officinalis Polysaccharides Ethanol fractional precipitation Antioxidant Antitumor |
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