Determination of the degree of N-acetylation (DA) of chitin and chitosan in the presence of water by first derivative ATR FTIR spectroscopy |
| |
Authors: | Stephan BeilAnna Schamberger Wolfgang NaumannSusanne Machill Karl-Heinz van Pée |
| |
Institution: | a Allgemeine Biochemie, TU Dresden, Bergstrasse 66, D-01062 Dresden, Germany b Bioanalytische Chemie, TU Dresden, Bergstrasse 66, D-01062 Dresden, Germany |
| |
Abstract: | A new method for the determination of the degree of N-acetylation (DA) of chitin and chitosan is described using first derivative diamond ATR FTIR spectroscopy. Applying the derivative values of the amide III band at 1327 cm−1 and the CH deformation band of the N-acetyl group at 1383 cm−1 as measure of the N-acetyl content of the sample in relation to the derivative value of the bridge oxygen vibration at 1163 cm−1 as internal standard, a linear correlation to the results of first derivative UV spectroscopy was obtained and confirmed by elemental analysis and Raman spectroscopy. The described method allows the determination of the degree of N-acetylation of chitosan and chitin in the presence of water thus making drying procedures unnecessary. |
| |
Keywords: | Chitosan Chitin Degree of N-acetylation First derivative ATR FTIR spectroscopy |
本文献已被 ScienceDirect 等数据库收录! |