Exoerythrocytic Development of Plasmodium Gallinaceum Sporozoites In A Chicken Fibroblast Cell Line and Inhibition of the Cell Invasion By Specific Anti-Sporozoite Monoclonal Antibodies |
| |
| Authors: | ELIANA M M ROCHA MICHAEL R HOLLINGDALE ROBERT GWADZ ANTONIANA U KRETTLI |
| |
| Institution: | Department of Parasitology, ICB, Federal University of Minas Gerais and RenéRachou Research Center, FIOCRUZ, Belo Horizonte, 30190, Minas Gerais, Brazil,;Biomedical Research Institute, Rockville, Maryland, USA;National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland, USA |
| |
| Abstract: | ABSTRACT. Cultivation of the Plasmodium gallinaceum exoerythrocytic forms from sporozoites was attempted in three diferent cell lines: HEPG2-A16 (from a human hepatoma), VERO (monkey kidney epithelial cells) and SL-29 (chicken embryo fibroblast cells). the sporozoites in vaded all three cell types but their development into exoerythrocytic forms ocurred only in the SL-29 cells. In the presence of specific monoclonal antibodies against the major circumsporozoite protein, there were varying degrees of inhibition of parasite invasion of the SL-29 cells. of seven monoclonal antibodies tested, two completely inhibited cell invasion at high concentrations and caused intense inhibition at concentrations as low as 2.5 μg/ml, four caused intense inhibition at these various concentrations, and one had no effect on sporozoite invasion. |
| |
| Keywords: | SL-29 cells sporozoite infectivity antibody inhibition exoerythrocytic forms |
|
|
