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Antibiotic resistance gene cassettes derived from the π interposon for use in E. coli and Streptomyces |
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| Authors: | Marie-H l ne Blondelet-Rouault Jaroslav Weiser Ahmed Lebrihi Pavel Branny Jean-Luc Pernodet |
| Affiliation: | a Institut de Génétique et Microbiologie, URA CNRS 2225, Bât. 400, Université Paris XI, F-91405, Orsay, France b Institute of Microbiology, Czech Academy of Sciences, Videnská 1083, 14200, Prague 4, Czech Republic c Laboratoire de Microbiologie Industrielle, ENSAIA, Institut National Polytechnique de Lorraine, 2, Avenue de la Forêt de Haye, F-54500, Vandoeuvre, France |
| Abstract: | Three antibiotic resistance gene cassettes, derived from the π interposon (Prentki and Krisch (1984) Gene 29, 303–313) were constructed. These cassettes carry different antibiotic resistance genes, conferring resistance to geneticin, hygromycin or viomycin, flanked by short inverted repeats containing transcription and translation termination signals and synthetic polylinkers. These cassettes were designated πaac, πhyg and πvph. Resistance phenotypes conferred by these constructions are selectable in E. coli and Streptomyces. These cassettes can be used for insertional mutagenesis or for vector construction. |
| Keywords: | Aminoglycoside acetyltransferase Hygromycin phosphotransferase Viomycin phosphotransferase |
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