Production of copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate by fed-batch culture of Alcaligenes SP. SH-69 |
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| Authors: | Y H Rhee J-H Jang P L Rogers |
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| Institution: | (1) Department of Biotechnology, University of New South Wales, 2033 Kensington, NSW, Australia;(2) Department of Microbiology, Chungnam National University, 305-764 Daejeon, Korea |
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| Abstract: | Summary Production of copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate poly(3HB-co-3HV)] by fed-batch culture of Alcaligenes sp. SH-69 was investigated using glucose as a sole carbon source. Synthesis of poly(3HB-co-3HV) during the polymer accumulation stage was favored under dissolved oxygen tension at 20% and C/N ratio (mol glucose/mol ammonium) of 23.1. When conditions were optimal, 36 g liter-1 of poly(3HB-co-3HV) containing 3.0 mol% of 3HV was produced. Decreasing C/N ratio resulted in an increase of 3HV fraction in the copolymer to a maximum level of 6.3 mol%. |
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