Dictyosome-like structures from guinea-pig testes lack galactosyltransferase,a Golgi apparatus marker |
| |
| Authors: | Prof D James Morré Gary R Matyas Hilton H Mollenhauer |
| |
| Institution: | (1) Department of Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, Indiana, USA;(2) Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA;(3) Department of Agriculture, Veterinary Toxicology and Entomology Research Laboratory, College Station, Texas, USA;(4) Department of Medicinal Chemistry and Pharmacognosy, Pharmacy Building, Purdue University, 47907 West Lafayette, IN, USA |
| |
| Abstract: | Summary More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal enzymes arylsulfatase and latent acid phosphatase were present in the DLS fractions as were the Golgi apparatus activities thiamine pyrophosphatase and nucleoside diphosphatase. The presence of the latter two enzymes in DLS, plus NADH-ferricyanide reductase, has been verified from cytochemistry. On the other hand, the Golgi apparatus marker, galactosyltransferase, was not enriched in DLS fractions and appeared to be absent. This latter finding, verified from cytochemistry with isolated DLS fractions and, in situ, from 3H]galactose incorporation by testis tubules with analysis by autoradiography, provides the first clear biochemical characteristic that serves unequivocally to distinguish DLS from conventional Golgi apparatus.Work supported in part by a grant from the National Institutes of Health HD 11508 |
| |
| Keywords: | Dictyosome-like structures Galactosyltrans-ferase Golgi apparatus Testis Guinea pig |
| 本文献已被 SpringerLink 等数据库收录！ |
|
