Identification of a type III secretion system in uropathogenic Escherichia coli |
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Authors: | Miyazaki Jun Ba-Thein William Kumao Toshio Akaza Hideyuki Hayashi Hideo |
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Institution: | Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba-shi, Ibaraki 305-8575, Japan. md995454f@md.tsukuba.ac.jp |
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Abstract: | To determine virulence-related genes in uropathogenic Escherichia coli (UPEC) showing invasiveness to T-24 bladder cancer cells, genomic subtractive hybridization was performed between a highly invasive and a less invasive strain. Forty-nine DNA fragments were isolated from the invasive strain. One of them showed homology with Salmonella invA gene. By chromosomal walking of the strain, a type III secretion system that has been described in E. coli O157:H7 was identified on the genome of the invasive strains. Three strains out of 100 UPEC isolates had a type III secretion system inserted at 64 min of the chromosome, corresponding to E. coli K-12 MG1655. This finding suggested that the type III secretion system could play a part in uropathogenicity of UPEC. |
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Keywords: | Uropathogenic Escherichia coli Genomic subtractive hybridization Invasion Type III secretion system |
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